NAD+ synthase (glutamine-hydrolysing)

NAD+ synthase (glutamine-hydrolyzing)
Identifiers
EC number 6.3.5.1
CAS number 37318-70-0
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

In enzymology, a NAD+ synthase (glutamine-hydrolysing) (EC 6.3.5.1) is an enzyme that catalyzes the chemical reaction

ATP + deamido-NAD+ + L-glutamine + H2O \rightleftharpoons AMP + diphosphate + NAD+ + L-glutamate

The 4 substrates of this enzyme are ATP, deamido-NAD+, L-glutamine, and H2O, whereas its 4 products are AMP, diphosphate, NAD+, and L-glutamate.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor. The systematic name of this enzyme class is deamido-NAD+:L-glutamine amido-ligase (AMP-forming). Other names in common use include NAD+ synthetase (glutamine-hydrolysing), nicotinamide adenine dinucleotide synthetase (glutamine), desamidonicotinamide adenine dinucleotide amidotransferase, and DPN synthetase. This enzyme participates in glutamate metabolism and nicotinate and nicotinamide metabolism.

Structural studies

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes 1EE1, 1FYD, 1IFX, 1IH8, 1KQP, 1NSY, and 2NSY.

References